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Anti-MAP1A/MAP1B LC3 Mouse Monoclonal Antibody [clone: BS405]
Anti-MAP1A/MAP1B LC3 Mouse Monoclonal Antibody [clone: BS405]
Catalog # BSENM-1725-100
Supplier:  Biosensis
Anti-MAP1A/MAP1B LC3 Mouse Monoclonal Antibody [clone: BS405]
Catalog # BSENM-1725-100
Supplier:  Biosensis

Specifications

  • Antibody type:
    Primary
  • Antigen name:
    Microtubule-associated proteins 1A/1B light chain 3A
  • Antigen symbol:
    MAP1A/MAP1B LC3
  • Clonality:
    Monoclonal
  • Clone:
    BS405
  • Conjugation:
    Unconjugated
  • Flow cytometry:
    Yes
  • Host:
    Mouse
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG2a kappa
  • Reactivity:
    Human,
    Rat
  • Western blot:
    Yes
  • Epitope:
    A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.
  • Format:
    Protein G purified IgG.
  • Form:
    Lyophilized
  • Gene ID:
    Q9H492 MLP3A_HUMAN
  • Antigen synonyms:
    MAP1 light chain 3-like protein 1|MAP1A/MAP1B LC3A|Autophagy-related ubiquitin-like modifier LC3 A|MAP1LC3A|APG8a|Microtubule-associated protein 1 light chain 3 alpha|Autophagy-related protein LC3 A|MAP1A/1B light chain 3 A
  • Storage buffer:
    PBS, pH 7.4, with 3% Trehalose, without preservatives
  • Sequence:
    C-RSFADRCKEVQQI
  • Storage temperature:
    After reconstitution divide in to aliquots and store at −20 °C for a higher stability. Storage at 4 °C with an appropriate antibacterial agent.
  • Shipping temperature:
    Room (ambient) temperature.
  • Immunogen:
    A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.
  • Purification:
    Protein G purified.
  • Size:
    100 ug
  • Pk:
    100 µG

Specifications

About this item

MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.

Flow Cytometry (20 µg/mL), Western blot (10 µg/mL), Immunocytochemistry (1-2 µg/mL). Other applications have not yet been tested. Biosensis recommends optimal dilutions and concentrations should be determined by the end user.

Type: Primary
Antigen: MAP1A/MAP1B LC3
Clonality: Monoclonal
Clone: BS405
Conjugation: Unconjugated
Epitope: A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.
Host: Mouse
Isotype: IgG2a kappa
Reactivity: Human, Rat