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Anti-GAPDH Rabbit Polyclonal Antibody

Fornitore: Thermo Scientific
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: antigen standard for GAPDH (transient overexpression lysate). GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

Type: Primary
Antigen: GAPDH
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Primates, Rat, Mouse, Human



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Taglia Conf Codice catalogo VWR Quantità
100 µL 100 µl PIEAPA116777 Blocked
Codice articolo fornitore:: PA116777
Codice articolo locale:: PIEAPA116777
Blocked Questo prodotto è stato bloccato dalla vostra organizzazione. Si prega di contattare il vostro responsabile acquisti per ulteriori informazioni.

Specifiche

Antigene GAPDH
Nome antigene Glyceraldehyde-3-phosphate dehydrogenase
Reattività Primates, Mouse, Human, Rat
Coniugazione Unconjugated
Clonabilità Polyclonal
Tipo anticorpo Primary
Host Rabbit
Isotipo IgG
Western Blot Yes
Immunochimica Yes
Immunofluorescenza Yes
Citometria a flusso Yes
Sinomini antigene G3PD, G3PDH, Glyceraldehyde-3-Phosphate Dehydrogenase, GAPD
ID gene 14433, 24383, 2597
Concentrazione 0.2 mg/ml
Immunogeno Residues between 150 and 200 of human Glyceraldehyde-3-Phosphate Dehydrogenase (GeneID 2597)
Purificazione Antigen affinity chromatography
Forma Liquid
Tampone di conservazione TBS, 0.1% BSA, 0.09% sodium azide
Temperatura di conservazione 4° C, do not freeze
Temperatura di spedizione Ice
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