AccuBlue® and AccuClear® dsDNA Quantitation Kits offer sensitive and selective detection of double-stranded DNA in a 96-well microplate format.
The AccuBlue™ Broad Range assay has a wide dynamic range of 2 to 2000 ng per well of a 96 well plate (0,2 to 200 ng/µl of sample). The standard curve can be extended to 4000 ng/well with slight compromise in linearity. Excitation/emission wavelengths: 350/460 nm (blue fluorescence).
- Allow accurate quantitation of purified double-stranded DNA (dsDNA) samples across a wide range of concentrations
- Compatible with plate readers and handheld fluorometers
AccuBlue® high sensitivity assay: Non-toxic and non-mutagenetic DNA quantitation dye
The AccuBlue® high sensitivity assay has a linear range of 0,2 to 100 ng dsDNA per well of a 96 well plate (0,02 to 10 ng/µl of sample). Excitation/emission wavelengths: 500/530 nm (green fluorescence).
- High sensitivity
- Broad dynamic range
- Compatible with plate readers and handheld fluorometers
- Insensitive to ssDNA, RNA, dNTP's and protein
- Choice of 3 kits to best suit individual requirements
AccuClear® ultra high sensitivity assay: Versatile kit with high sensitivity and wide linear range
The AccuClear® ultra high sensitivity assay has a linear range of 30 pg to 250 ng dsDNA per well of a 96-well plate. Excitation/emission wavelengths: 460/507 nm (green fluorescence).
Note: AccuBlue® NextGen assay for quantitation of ultra-low amounts of DNA in precious or dilute samples is also available (see Cat. No. 31060). The AccuBlue® NextGen assay has a linear range of 1 pg to 3 ng (dependent on instrument sensitivity) dsDNA per well of a 96-well plate (0,5 pg/µl to 300 pg/µl of sample). Excitation /emission wavelengths: 468/507 nm (green fluorescence).
Informazioni per l'ordine: AccuBlue®and AccuClear® kits include quantitation solution and calf thymus dsDNA standards. Stand-alone AccuBlue® and AccuClear® quantitation solutions are available for users who prefer to use their own DNA standards.